Au-FLAT gold support films
Au-FLAT™ holey grids are a premier ultra-flat holey gold foil support film perfectly suited for the highest resolution imaging in cryo-TEM and cryo-electron tomography.
Au-FLAT is an ultrastable Cryo-EM sample support with a 45nm holey Gold alloy film on 3.05 mm gold mesh grids. Au-Flat is a derivative of the patented C-Flat grid.
Au-FLAT have a Au/Pd support film with a regular pattern of holes on a gold grid that reduces movement during hi-resolution imaging compared to holey carbon support films. The perforated gold support film has pre-defined hole size, shape and arrangement. Available in the popular 1.2/1.3 and 2/2 versions.
See ordering information below.
Better reconstructions with less data
Au-FLAT significantly reduces beam-induced motion during imaging compared to carbon films, improving image quality and resolution.
Au-FLAT features a holey Au/Pd film on a gold mesh grid, so it’s chemically inert and biologically compatible.
Au-FLAT support film is significantly stronger than other films and is more capable of surviving the cryoEM workflow including tweezer handling, plasma treatment, glow discharge, blotting, plunge freezing, and autogrid loading.
The lighter color of the Au/Pd film compared to the underlying gold mesh grid makes it easy to identify the “holey” side of the grid during sample preparartion. This ensures the sample is deposited on the correct side and that the grid is loaded and imaged in the correct orientation.
Just like our C-Flat grids, Au-FLAT is readily available from Labtech-EM
The 1.2/1.3 version is available in a small pack size of five for those wishing to evaluate before committing to a larger quantity.
Protochips has conducted extensive field trials to test and validate the benefits of the Au-FLAT. These trials were conducted by researchers at New York Structural Biology Center, Caltech, CUNY, and Duke University.
Feedback from these users:
“They’re quite fantastic!”
“Great drift results!”
“…almost all the pictures we took were reaching between 2.9 and 3.1Å resolution based on CTF thon rings. This is amazingly consistent, probably much more than with any other dataset I’ve collected.”
“… shows reduced overall motion and max in-frame motion.”
“They perform comparably to (other gold-foil TEM grids)”
“It is much easier to see the holey film side of the Au-Flat grid. This is significant, because it is very difficult with (other gold foil TEM grids), where the sides look identical.”
“No difference (compared to other Cryo-grids) when preparing the grids.”
“Ice thickness uniformity is similar to other grids.”
“Particles are uniformly distributed across the grid.”
“The grids feel sturdier.”