CryoCapCell HPM Live high pressure freezer
The CryoCapCell HPM Live μ is an advanced, automated high pressure freezer for the vitrification of specimens. HPM Live μ can be used for the vitrification of a wide range of cell and tissue types - a range of holders enables selection of the optimal holder for each specimen type. HPM Live μ is the only existing commercial solution to enable live Correlative Light and Electron Microscopy (CLEM) experiments.
CLEM aims at combining functional information obtained from live cell imaging by light microscopy with structural information only possible with electron microscopy. CLEM is a challenging technique where each single step is crucial. Revealing life is often a matter of seconds.
Rapid transfer in 1.26 seconds
CLEM by high pressure freezing enables researchers to go beyond human speed and relate a live event to its ultrastructure. HPM Live μ is a unique tool that optimises the entire workflow of CLEM with vitrification by high pressure freezing. At the heart of the process is the CryoCapsule which comprises a special sapphire disc printed with carbon coordinates and a gold spacer ring maintained together by a polyethylene ring. The CryoCapsule is entirely harmless to biological material and protects the specimen against mechanical damage during sample preparation.
Class-leading high pressure freezing
HPM Live µ is the first high pressure freezer that can be interfaced to a light microscope to allow the specimen to be examined live before vitrification. HPM Live µ is designed for use with CryoCapsule to give the fastest available live cell imaging to vitrification solution. HPM Live µ integrates an automated specimen arm with a light microscope by holding the CryoCapsule containing the specimen above the objective. At the appropriate time the arm is activated and in less than two seconds the specimen is vitrified.
CLEM workflow with CryoCapsule
With CryoCapsule all manipulations are done using a plastic ring, which prevents scratching of the sapphire surface containing the biological specimens. A 50um thick gold spacer ring creates a cavity to prevent unwanted pressure or shear forces on the cells before vitrification by high pressure freezing. In comparison to the conventional sapphire disc manipulation, the number of steps is reduced from 8 to 4.
See comparison in the diagram showing workflows (below)
HPM Live µ highlights:
- Automatic specimen transfer in 1.26 seconds
- Start-up cycle fully controlled by HPM Live μ
- High pressure freezing cycle lasts 20 minutes
- User defined parameters
- 15” inch HD colour touchscreen
- Data and curves are automatically saved and can be sent by e-mail
- Vitrification of two successive samples in 45 seconds
- Optimized workflow for CryoCapsule®
- Open system and flexible to use - also compatible with a range of sample carriers
- Remote control and online diagnostics if connected to internet