LumaScopes
LumaScopes are a new concept in high resolution, inverted fluorescence microscopy designed to speed up workflow in cell biology. They are compact, highly versatile microscopes that provide high resolution fluorescence images comparable in quality to traditional higher cost instruments. Their compact size enables them to work in the challenging locations used in cell biology such as inside incubators, biological safety cabinets and in environmental control workstations with remote control and image monitoring.
The LumaScope design was conceived and developed by working scientists to simplify daily work and overcome the need for expensive and overly complicated microscopes. They realised that a large proportion of routine fluorescence microscopy involves imaging and measuring the presence, health, and signal of labelled cells that could be greatly simplified. The inverted design enables rapid imaging of microplates, flasks, dishes and custom labware in addition to slides. Applications include:
- Live cell imaging
- Cell growth and confluence
- Cell migration and wound healing
- Determining transfection efficiency
- Tracking and quantifying protein expression
- Following cell differentiation
- High Content Analysis
- Intravital studies
- Photomicroscopy in locations without AC power
LumaScopes use LED light sources, Semrock filters combined with advanced optical engineering and CMOS sensors to provide near diffraction-limited resolution. Powered only by a USB connection, LumaScopes easily record still images, time-lapse series, and live videos directly to your computer. The XY Stage on the LumaScopes 460, 560 or 620 can be operated manually or fully automated on the LumaScope 720.
LumaScopes focus on producing quality images whilst providing versatility in a compact, easy to use design at a very attractive price. Lumaview software sends images directly to a computer via a USB cable, eliminating the need for on-board image storage and processing. Lumaview enables time-lapse studies over seconds, minutes, hours, or days. Live videos can be recorded at up to 30 frames per second.
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Comparison of Lumascope Models |
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Click model name for more information >>> |
Lumascope 560 |
Lumascope 620 |
Lumascope 720 |
Optics |
Green fluorescence; brightfield |
Blue, green & red fluorescence; brightfield |
Blue, green & red fluorescence; brightfield |
Phase Contrast |
Phase contrast optional (Olympus) |
Phase contrast optional (Olympus) |
Phase contrast optional (Olympus) |
Objective Options |
1.25x,2.5x, 4x, 10x, 20x, 40x, 60x, and 100x(oil) magnification |
1.25x,2.5x, 4x, 10x, 20x, 40x, 60x, and 100x(oil) magnification |
1.25x,2.5x, 4x, 10x, 20x, 40x, 60x, and 100x(oil) magnification |
Objective Compatibilities |
RMS-threaded, infinity corrected, 45 mm parfocal distance |
RMS-threaded, infinity corrected, 45 mm parfocal distance |
RMS-threaded, infinity corrected, 45 mm parfocal distance |
Fluorescence Filters |
Green: Ex 457-493, Em 508-552 nm |
Blue: Ex 370-410, Em 429-462 nm; Green: Ex 473-491, Em 502-561 nm; Red: Ex 580-598, Em 612-680 nm |
Blue: Ex 370-410, Em 429-462 nm; Green: Ex 473-491, Em 502-561 nm; Red: Ex 580-598, Em 612-680 nm |
Camera |
High Sensitivity Monochrome CMOS Sensor; C-mount |
High Sensitivity Monochrome CMOS Sensor; C-mount |
High Sensitivity Monochrome CMOS Sensor; C-mount |
Image Formats |
JPG, BMP, TIF, or PNG |
JPG, BMP, TIF, or PNG |
JPG, BMP, TIF, or PNG |
Image Size |
100x100 to 1700x1700 pixels |
100x100 to 1900x1900 pixels |
100x100 to 1900x1900 pixels |
Field of View |
Up to 0.78 x 0.78 mm at 20x |
Up to 0.78 x 0.78 mm at 20x |
Up to 0.78 x 0.78 mm at 20x |
Video Rates |
Up to 10 fps; with reduced frame size, up to 30 fps |
Up to 10 fps; with reduced frame size, up to 30 fps |
Up to 10 fps; with reduced frame size, up to 30 fps |
Stage |
Manual XY |
Manual XY |
Automated XYZ, SBS nest, 6- to 1536-well microplates; microfluidic chambers (contact Etaluma) |
Subdeck |
NA |
NA |
No automation; most flasks, dishes, other |
Well-to-well time (seconds) |
NA |
NA |
Image 96 wells: a) 1 colour focus & image: 13 min; b) 3 colour focus & image: 34 min |
Computer Requirements |
Windows 7, 8, 8.1, 10; Core i5, SSD |
Windows 7, 8, 8.1, 10; Core i5, SSD |
Windows 7, 8, 8.1, 10; Core i5, SSD |
Automation Friendly |
SDK available |
SDK available |
SDK available |
Power Requirements |
USB |
USB |
USB for LS; 100-240 V, 50-60 Hz for auto stage |
Dimensions (WxDxH) |
24 x 14 x 16.5 cm |
24 x 14 x 16.5 cm |
37.4 x 43.8 x 23.9 cm |
Weight |
3.25 kg |
3.25 kg |
11 kg |
Operating Conditions |
0°C - 42°C, 5% - 95% RH non-condensing |
0°C - 42°C, 5% - 95% RH non-condensing |
0°C - 42°C, 5% - 95% RH non-condensing |