Correlative light and electron microscopy (CLEM) is a powerful technique for localising dynamic events in cells with light microscopy and combining it with the high resolution ultrastructure from TEM.
Our colleagues at CryoCapCell have been making some significant progress in improving the CLEM workflow. There have been some very interesting results using CryoCapsule at Nico Sommerdijk's facility in Nijmegen together with Anat Akiva. By culturing cells directly in the carriers (in which the cells grow very happily), they could image them live at 10X with Zeiss airyscan mode, HPF and then with either viewing on a Linkam CryoStage or after Freeze Substitution using the new R221 resin. EM results will follow hopefully soon after Covid 19 confinement to finalize the figures. Read about the findings so far.
Labtech-EM offers a range of instrumentation and consumables used in a CLEM workflow. Please click on the following links for more information:
- High pressure freezing with the HPM u live
- Freeze substitution with the FS8500
- Resin embedding with R221 resin
- Resin sectioning with a RMC PowerTome
- Perfect sections with DiATOME diamond knives